【Abstract】 Objective To explore the value of isozyme zymograms in the classification of blackflies and accumulate basic biochemical data for the study of phylogenesis. Methods EST isozymes of three blackfly species were studied by discontinuous PAGE techniques. Results Ten isozyme bands could be identified according to the electrophoresis zymograms. The differences of zymograms were significant between three blackfly species and each species had its own special zymogram. The main band among intraspecific individuals or between the larvae and pupae was alike， however， other enzyme bands were different. Conclusion EST isozyme zymograms could be used as one of the classification characteristics of blackflies.

Objective To carry out surveillance on the density and breeding of vector for controlling and preventing of Dengue fever in Wuyishan City.Methods The species and breeding of vector were investigated and the density was caculated by Breteau index in 2001.Results Only Aedes albopictus was found in the localiy.The Breteau index were 15.91 in the airport and 8.90 in the Shangu Holiday region and 53.26 in two villages respectevily.It was five times in the old village of that in the new villages.The small containers were the main breeding habitats for Aedes albopictus,such as snack boxes and the electrical equipment packages in the airport,a large number old jars in villages,tyres and rockery saving water in Shangu Holiday region.Conclusion The density of Aedes albopictus in the old village was higher than that in the other environments such as new village,airport,holiday region in Wuyishan City.